Speciesspecific PCR primers for <i>Guignardia citricarpa</i> and <i>Guignardia mangiferae</i>

PCR primers and conditions for nonmarine ostracods.

1.Blaseio, U. and F. Pfeiffer. 1990. Transformation of Halobacterium halobium: development of vectors and investigation of gas vesicle synthesis. Proc. Natl. Acad. Sci. USA 87:67726776. 2.Cline, W.S., W.L. Lam, R.L. Charlebois, L.C. Schalkwyk, and W.F. Doolittle. 1989. Transformation methods for halophilic archaebacteria. Can. J. Microbiol. 35:148-152. 3.Cohen, S.N., A.C.Y. Chang, and L. Hsu. 1...

Designing PCR Primers Painlessly†

PCR with arbitrary primers

Evaluation of PCR primers for cytomegalovirus detection.

We read with great interest the article by Mendez et al. (2) published in a recent issue evaluating different PCR primers for cytomegalovirus (CMV) detection. As CMV PCR becomes a frequently used assay in the management of immunocompromised patients, such as transplant recipients and human immunodeficiency virus-infected individuals, an increasing number of laboratories are establishing in-hous...

Selecting Optimal Oligonucleotide Primers for Multiplex PCR

We investigate the problem of designing efficient multiplex PCR for medical applications. We show that the problem is NP-complete by transformation to the Multiple Choice Matching problem and give an efficient approximation algorithm. We developed this algorithm in a computer program that predicts which genomic regions may be simultaneously amplified by PCR. Practical use of the software shows ...